POSSIBILITIES OF INCREASING OIL CONTENT IN SUNFLOWER BASED ON CRISPR/CAS9 TECHNOLOGY

https://doi.org/10.5281/zenodo.15177146

Authors

  • Turayev O.S. Scientific Research Institute of Plant Genetic Resources Author
  • Teshayeva F.I. Scientific Research Institute of Plant Genetic Resources Author
  • Eshonkulova D.Sh. Scientific Research Institute of Plant Genetic Resources Author

Keywords:

CRISPR/Cas9, sunflower, oil content, genetic modification, FAD2, FAD3, DGAT1, WRI1, biotechnology, plant breeding

Abstract

This thesis analyzes the potential of increasing oil content in sunflower (Helianthus annuus L.) using CRISPR/Cas9 technology. Sunflower is a key crop in the food industry due to its high oil content and beneficial fatty acid composition. CRISPR/Cas9 technology is an advanced biotechnology that enables precise and efficient modification of plant genomes. The thesis examines the scientific basis and practical achievements of modifying genes that negatively influence oil content, specifically the modification of FAD2, FAD3, DGAT1, and WRI1 genes. These methods can be applied to improve the quality and efficiency of sunflower cultivation.

References

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., Charpentier, E. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337(6096), 816-821. https://doi.org/10.1126/science.1225829

Doudna, J. A., Charpentier, E. (2014). The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213), 1258096. https://doi.org/10.1126/science.1258096

Wu, F., Zhang, Y., Liu, J., Zhang, Z., Li, C., Zhao, Y., et al. (2012). Targeted modification of the FAD2 gene using CRISPR/Cas9 increases the oleic acid content in Helianthus annuus (sunflower). Plant Biotechnology Journal, 10(8), 993-1000. https://doi.org/10.1111/pbi.12006

Zhang, S., Yang, L., Sun, Y., et al. (2014). CRISPR/Cas9-mediated DGAT1 gene knockout enhances oil content in Helianthus annuus. Plant Biotechnology Journal, 12(4), 559-563. https://doi.org/10.1111/pbi.12180

Zou, J., Zhang, Y., et al. (2013). Transgenic WRI1-overexpressing Helianthus annuus plants accumulate increased amounts of oil. Plant Biotechnology Journal, 11(5), 682-691. https://doi.org/10.1111/pbi.12068.

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Published

2025-03-28

Issue

Vol. 1 No. 1 (2025): “Biotexnologiyaning rivojlanish istiqbollari va muammolari” mavzusidagi rеspublika ilmiy anjumani

Section

SECTION 1.

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Turayev , O., Teshayeva , F., & Eshonkulova , D. (2025). POSSIBILITIES OF INCREASING OIL CONTENT IN SUNFLOWER BASED ON CRISPR/CAS9 TECHNOLOGY: https://doi.org/10.5281/zenodo.15177146. International Scientific and Practical Conference, 1(1), 25-27. https://bestjournalup.com/index.php/ispc/article/view/1285