POLIMERAZA ZANJIR REAKSIYASI UCHUN REKOMBINANT VENT POLIMERAZA FERMENTINING ANALOGINI OLISH

https://doi.org/10.5281/zenodo.15176513

Authors

  • U.A.Davlatboeva Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston Author
  • I.I.Karimov Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston Author
  • M.N.Naubetova Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston Author
  • M.I.Muminov O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston Author
  • Sh.N.Qo’ziyev O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston Author
  • Sh.U.Turdikulova Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston Author

Abstract

Molekulyar biologiya fanida polimerazalar asosiy fermentlar sifatida tan olingan bo‘lib, ular DNK va RNK sintezini amalga oshirishda muhim o‘rin egallaydi. DNK polimerazalari hujayralardagi matritsa DNK replikatsiyasi, ta'miri va rekombinatsiya jarayonlarini bevosita ishtirok etadi [1]. Ushbu fermentlarning biologik jarayonlardagi vazifalari qanchalik muhim bo‘lsa, ularning laboratoriya sharoitida DNK polimerazalarining yuqori haroratga chidamli turlari polimeraza zanjiri reaksiyasi (PZR) kabi texnologiyalarni amalga oshirishda katta ahamiyatga ega. Polimeraza zanjirli reaksiyasi DNK molekulalarini ko‘paytirish uchun keng qo‘llaniladigan texnologiya bo‘lib, u 1985-yilda Keri Mullis tomonidan ixtiro qilingan [2]. PZR yordamida atigi bir necha nusxada bo‘lgan DNK fragmentlari millionlab nusxaga ko‘paytirish mumkin. Ushbu texnologiya inson genomini to‘liq o‘rganishga qaratilgan yirik ilmiy tashabbus-Inson Genomi Loyihasi (Human Genome Project) doirasida muhim vosita sifatida qo‘llanilgan. Ushbu usul genlarni klonlash, sekvenslash, klinik diagnostikada, sud-tibbiy ekspertizasida, va turli boshqa molekulyar biologiya sohalarida katta muvaffaqiyat bilan qo‘llanilmoqda.

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Author Biographies

  • U.A.Davlatboeva, Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston

    O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

  • M.N.Naubetova, Ilg’or texnologiyalar markazi, 100174 Toshkent, O’zbekiston

    O’zMU Biologiya va ekologiya fakulteti, 100174 Toshkent, O’zbekiston

References

Lehman, I. R., & Kaguni, L. S. (1989). DNA polymerase α. Journal of Biological Chemistry, 264(8), 4265–4268. https://doi.org/10.1016/S0021-9258(18)83733-4

Kornberg, A. (1988). DNA replication. Journal of Biological Chemistry, 263(1), 1–4. https://doi.org/10.1016/S0021-9258(19)57345-8

Mullis, K. B. (1990). The Unusual Origin of the Polymerase Chain Reaction. ScientificAmerican,262(4),56–65. https://doi.org/10.1038/scientificamerican0490-56

François Vigneault and Régen Drouin. 2005. Optimal conditions and specific characteristics of Vent exo– DNA polymerase in ligation-mediated polymerase chain reaction protocols. Biochemistry and Cell Biology. 83(2): 147-165. https://doi.org/10.1139/o04-134

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Published

2025-03-28

Conference Proceedings Volume

Vol. 1 No. 1 (2025): “Biotexnologiyaning rivojlanish istiqbollari va muammolari” mavzusidagi rеspublika ilmiy anjumani

Section

SECTION 1.

License

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Davlatboeva, U., Karimov, I., Naubetova, M., Muminov, M., Qo’ziyev, S., & Turdikulova, S. (2025). POLIMERAZA ZANJIR REAKSIYASI UCHUN REKOMBINANT VENT POLIMERAZA FERMENTINING ANALOGINI OLISH: https://doi.org/10.5281/zenodo.15176513. International Scientific and Practical Conference, 1(1), 19-22. https://bestjournalup.com/index.php/ispc/article/view/1283